Proinflammatory effects of Staphylococcus aureus enterotoxin B on human nasal epithelial cells / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 202-206, 2007.
Article
em Zh
| WPRIM
| ID: wpr-262812
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of Staphylococcus aureus enterotoxin B (SEB) on proinflammatory cytokine/chemokine releases in primary human nasal epithelial cell (HNEC).</p><p><b>METHODS</b>Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of SEB 1, 10, 100 ng/ml, IL-1beta 20 ng/ml and SEB 10 ng/ml + dexamethasone 13 ng/ml for 12,24 and 48 h, respectively. The expression of IL-5 and Granulocyte-macrophage colony stimulating factor (GM-CSF) mRNA derived from epithelial cells was detected by in situ hybridization.</p><p><b>RESULTS</b>(1) The expression of IL-5 and GM-CSF mRNA was time and dose-dependent, and reached to a peak under SEB 10 ng/ml for 24 h (P < 0.05). The mRNA expressed more intensively in epithelial cells from NP than IT (P < 0.05). (2) The expression of IL-5 and GM-CSF mRNA increased less under the stimulus of IL-1beta than SEB 10 ng/ml (P < 0.05). (3) The mRNA level of IL-5 and GM-CSF decreased under the stimulus of SEB + dexamethasone 13 ng/m when compared with the stimulus of SEB 10 ng/ml (P < 0.05).</p><p><b>CONCLUSION</b>SEB showed proinflammatory effects on HNEC.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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RNA Mensageiro
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Dexametasona
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Células Cultivadas
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Fator Estimulador de Colônias de Granulócitos e Macrófagos
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Interleucina-5
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Biologia Celular
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Enterotoxinas
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Células Epiteliais
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2007
Tipo de documento:
Article