Expression and mutation of myc antagonist genes Mad1, Mxi1 and Rox in leukemia cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 745-749, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-262954
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and mutation of Mad1, Mxi1 and Rox genes in leukemia cells.</p><p><b>METHODS</b>Expression and mutation of Mad1, Mxi1 and Rox genes in bone marrow mononuclear cells (BMMNC) from 26 de novo acute leukemia (AL) patients, and in peripheral blood mononuclear cells (PBMNC) from 30 healthy volunteers, as well as in 7 human leukemic cell lines were analyzed by reverse transcription-polymerase chain reaction (RT-PCR), single strand conformational polymorphism (SSCP) and DNA sequencing.</p><p><b>RESULTS</b>RT-PCR showed that all the above cells expressed Mad1, Mxi1 and Rox mRNA. SSCP revealed four polymorphisms two in Mad1, one each in Mxi1 and Rox. DNA sequencing detected nine missense mutations two in Mad1 in AL patients, four in Mxi1 (three in AL patients and one in KG-1 cell line), and three in Rox in AL patients. The mutations of Mad1, Mxi1 and Rox mRNA were detected in 2, 3 and 3 patients, respectively.</p><p><b>CONCLUSION</b>It is for the first time to demonstrate the mutations of Mad1, Mxi1 and Rox genes in AL patients suggesting these mutated genes involve in the pathogenesis of leukemia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Repressoras
/
Proteínas Nucleares
/
Leucemia
/
Polimorfismo Conformacional de Fita Simples
/
Proteínas de Ciclo Celular
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Proteínas Supressoras de Tumor
/
Fatores de Transcrição Hélice-Alça-Hélice Básicos
/
Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos
/
Genética
/
Metabolismo
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo
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