The immunoregulatory function of bone marrow mesenchymal stem cells on allogeneic B lymphocytes / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 616-620, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-262972
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunoregulatory function of bone marrow mesenchymal stem cells (MSCs) on allogeneic B lymphocytes in vitro, and explore its possible mechanisms.</p><p><b>METHODS</b>Human MSCs were isolated from bone marrow and expanded in vitro. The purity of MSCs were identified with the spindle fibroblastic morphology by micro-photograph and the phenotype by flow cytometry. MSCs were irradiated with 20 Gy of gamma ray to abolish proliferative capacity. The change of activated B cells proliferative capability and apoptosis with or without MSCs were examined. The effect of MSCs on activated B cells proliferation was compared between transwell cultures and non-transwell cultures. The IgG, IgA and IgM productions of B cells and the immune molecules expression with or without MSCs were assessed. RESULTS (1) MSCs could not induce the proliferation of B lymphocytes, but could suppress LPS activated B lymphocytes proliferation. (2) With the number of MSCs increased, a dose-dependent inhibitory effect was observed in B cell proliferation. MSCs could not induce B cells apoptosis. The activated B cells proliferation with MSCs in transwell culture was decreased, suggesting that MSCs inhibition of B cells might be mediated both by cell-to-cell contact and soluble factors. (3) MSCs suppressed the IgG, IgA and IgM production of B cells, but not suppressed the immune molecules HLA-DR, CD40, CD80 and CD86 expression.</p><p><b>CONCLUSION</b>Bone marrow MSCs can suppress allogeneic B lymphocytes proliferation and its secretion in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células da Medula Óssea
/
Linfócitos B
/
Comunicação Celular
/
Células Cultivadas
/
Imunofenotipagem
/
Apoptose
/
Biologia Celular
/
Proliferação de Células
/
Alergia e Imunologia
/
Células-Tronco Mesenquimais
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo
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