Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 284-290, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-263608
ABSTRACT
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Leveduras
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
/
DNA Complementar
/
Técnicas do Sistema de Duplo-Híbrido
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2002
Tipo de documento:
Artigo
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