Screening of antimitotic portions from sea hare using conidia of Pyricularia Oryzae / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 53-55, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-263640
ABSTRACT
<p><b>OBJECTIVE</b>To apply conidia of Pyricularia Oryzae to the screening of antimitotic constituents from marine animal sea hare.</p><p><b>METHOD</b>To extract and fractionate active portions from sea hare through detecting deformation of mycelia germinated from conidia of P. Oryzae P-2b, in comparison with the cytotoxic test results in vitro.</p><p><b>RESULT</b>Two active portions, of which IC50 against P388 and HL-60 was 23.4, 18.6 and 19.4, 12.5 micrograms.ml-1, respectively, were screened from this animal.</p><p><b>CONCLUSION</b>This bioassay method was efficiently applied to the primary screening of antimitotic portions from marine animals for the first time. Being convenient, speedy and cheap, the screening model is suitable for the bioassay of active constituents from marine life.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Aplysia
/
Farmacologia
/
Fisiologia
/
Materia Medica
/
Células Tumorais Cultivadas
/
Leucemia P388
/
Química
/
Células HL-60
/
Fungos Mitospóricos
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2002
Tipo de documento:
Artigo
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