Study on genetic polymorphisms of CYP2F1 gene in Guangdong population of China / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 383-387, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-263772
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic polymorphism of CYP2F1 gene, a member of CYP450 gene family in the healthy population and the patients with nasopharyngeal carcinoma (NPC) of Guangdong province, and furthermore analyze the relationship between CYP2F1 genetic polymorphism and the risk of developing NPC.</p><p><b>METHODS</b>By direct gene sequencing, all of 10 exons of CYP2F1 gene were detected in 40 peripheral blood specimens of patients with primary NPC. For the genetic polymorphism with high allelic frequency, mismatch PCR-RFLP technique was developed to identify the different frequency between 368 NPC cases and 344 cancer-free controls.</p><p><b>RESULTS</b>There were totally 35 SNPs identified in all of 10 exons and exon-intron junctions of CYP2F1 gene from 40 NPC patients, which included 10 missense mutations and 1 frame shift mutation. The most important mutation was C insertion located in 15-16 bp, which caused the frame shift. The allelic frequency of C insertion was 25%. However, there was no significant difference found between 368 NPC cases and 344 controls in allelic frequency of 15-16 bp C insertion mutation (P>0.05).</p><p><b>CONCLUSION</b>A lot of genetic polymorphism of CYP2F1 gene is found in Guangdong population of China. However, no single genetic polymorphism associated with the individual susceptibility to NPC can be identified. The cooperated operations with multiple genetic polymorphisms of one or more genes may be critical factors contributing to the development and progression of NPC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Sequência de Bases
/
China
/
Neoplasias Nasofaríngeas
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Predisposição Genética para Doença
/
Polimorfismo de Nucleotídeo Único
/
Sistema Enzimático do Citocromo P-450
/
Povo Asiático
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2006
Tipo de documento:
Artigo
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