Distribution of MIF -173 single nucleotide polymorphism checked by tetra-primer amplification refractory mutation system / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 343-345, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-263779
ABSTRACT
<p><b>OBJECTIVE</b>To study the distribution of macrophagemigration inhibitory factor gene (MIF) -173 single nucleotide polymorphism (SNP) of Chinese Han population in Zhejiang province.</p><p><b>METHODS</b>The DNA samples were extracted from EDTA blood of 142 unrelated healthy individuals. Alleles of MIF -173 SNP were genotyped by using the techniques of tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR Meantime the PCR products were cloned and sequenced.</p><p><b>RESULTS</b>The authors detected three kinds of genotypes at the MIF -173 locus, and no deviation was observed from Hardy-Weinberg equilibrium. The final results were the same completely, whatever either tetra-primer ARMS or RFLP-PCR was used to check the MIF -173 single nucleotide polymorphism. Statistical analysis showed that the distributions of MIF -173 SNP alleles and genotype frequencies were significantly different between Chinese Han population and European Caucasian(P< 0.01), but no significant difference demonstrated to happen between Chinese and Japanese(P> 0.05).</p><p><b>CONCLUSION</b>Tetra-primer ARMS is an accurate, rapid and economical method for SNP genotyping.There exists ethnic difference in the distribution of MIF -173 SNP alleles.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Análise Mutacional de DNA
/
Dados de Sequência Molecular
/
Sequência de Bases
/
China
/
Reação em Cadeia da Polimerase
/
Fatores Inibidores da Migração de Macrófagos
/
Oxirredutases Intramoleculares
/
Polimorfismo de Nucleotídeo Único
/
Povo Asiático
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2006
Tipo de documento:
Artigo
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