Expression of circadian gene NPAS2 in colorectal cancer and its prognostic significance / 南方医科大学学报
Journal of Southern Medical University
;
(12): 714-718, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-263974
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of NPAS2 in colorectal cancer (CRC) and analyze its relationship with the clinicopathological parameters and prognosis of the patients.</p><p><b>METHODS</b>Real-time q-PCR was used to detect the expression of NPAS2 mRNA in 40 fresh CRC tissues and paired adjacent tissues; immunohistochemistry was used to detect the expression of NPAS2 protein in 120 paraffin-embedded tumor and adjacent tissues. The relationship between NPAS2 expression level and the 5-year survival rate of 78 CRC patients with follow-up data were analyzed with Kaplan-Meier survival analysis.</p><p><b>RESULTS</b>Compared with the adjacent tissues, fresh CRC tissue expressed significantly lower NPAS2 mRNA levels (P<0.01). Among the paraffin-embedded CRC tissues, 19.2% were positive for NPAS2 expression, as compared to a much higher rate of 62.5% in the adjacent tissues (P<0.05). The expression of NPAS2 was correlated with the tumor size, lymph node metastasis and TNM stages (P<0.05) but not with the patients' gender, age, distant tumor metastasis, differentiation, or invasion. Patients with high NPAS2 expression levels had a significantly higher 5-year survival rate than those with low NPAS2 expressions (P=0.0001).</p><p><b>CONCLUSION</b>NPAS2 is down-regulated in CRC and closely correlated with the malignant biological behavior of the tumor and 5-year survival of the patients, suggesting its value in predicting the prognosis of the CRC patients.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Prognóstico
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Neoplasias Colorretais
/
Regulação para Baixo
/
Taxa de Sobrevida
/
Diagnóstico
/
Fatores de Transcrição Hélice-Alça-Hélice Básicos
/
Estimativa de Kaplan-Meier
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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