Palmitic acid induces hepatocellular oxidative stress and activation of inflammasomes / 南方医科大学学报
Journal of Southern Medical University
;
(12): 655-659, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-263986
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of palmitic acid (PA) on oxidative stress and activation of inflammasomes in hepatocytes.</p><p><b>METHODS</b>To test the dose-dependent effect of PA on normal murine hepatocytes AML12, the cells were treated with 0, 0.15, 0.25 and 0.4 mmol/L of palmitic acid (PA). The cells were also divided into blank control group, 0.25 mmol/L PA group and 0.25 mmol/L PA+N-acetylcysteine (NAC) group to examine the effect of reactive oxygen species (ROS) on the activation of inflammasomes. After 24 h of treatment, lipid accumulation, total ROS, mitochondrial ROS, expression and localization of NOX4, and expressions of inflammasomes and IL-1β were detected in the hepatocytes.</p><p><b>RESULTS</b>Compared with the control cells, PA treatment of the cells significantly increased cytoplasmic lipid accumulation, concentrations of total ROS (12 463.09±2.72 vs 6691.23±2.45, P=0.00) and mitochondrial ROS (64.98±0.94 vs 45.04±0.92, P=0.00), and the expressions of NOX4, NLRP3, ASC, caspase-1, and IL-1β (1603.52±1.32 vs 2629.33±2.57, P=0.00). The mitochondria and NOX4 were found to be co-localized in the cytoplasm. NAC obviously reduced cellular ROS level stimulated by PA (7782.15±2.87 vs 5445.6±1.17, P=0.00) and suppressed the expressions of NLRP3, ASC and caspase-1.</p><p><b>CONCLUSION</b>PA treatment can stimulate lipid accumulation in hepatocytes and induce oxidative stress through NOX4 and mitochondria pathway to activate inflammasomes and stimulate the secretion of IL-1β.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Acetilcisteína
/
Proteínas de Transporte
/
Células Cultivadas
/
Espécies Reativas de Oxigênio
/
Estresse Oxidativo
/
Ácido Palmítico
/
NADPH Oxidases
/
Caspase 1
/
Hepatócitos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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