Your browser doesn't support javascript.
loading
Nanog promotes the invasion of breast cancer cells by increasing PKCε expression / 南方医科大学学报
Journal of Southern Medical University ; (12): 639-644, 2016.
Artigo em Chinês | WPRIM | ID: wpr-263989
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between Nanog-promoted metastasis of breast cancer and ezrin(T567) phosphorylation, and explore the possible mechanism by which Nanog regulates ezrin(T567) phosphorylation.</p><p><b>METHODS</b>A siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrin(T567) were detected using Western blotting and immunofluorescent staining; the protein interaction between PKCε and ezrin was assayed by co-immunoprecipitation and Western blotting.</p><p><b>RESULTS</b>Nanog knockdown significantly decreased the expression of PKCε protein, phosphorylation level of ezrin(T567) and the invasion ability of breast cancer cells. PKCε knockdown obviously decreased the phosphorylation level of ezrin(T567) in the cells, and PKCε and ezrin were co-immunoprecipitated.</p><p><b>CONCLUDIONS</b>Nanogcan can upregulate the expression of PKCε to promote the phosphorylation of ezrin(T567), which can be a new mechanism by which Nanog promotes tumor metastasis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fosforilação / Neoplasias da Mama / Células Tumorais Cultivadas / Transfecção / Regulação para Cima / Western Blotting / Proteínas de Homeodomínio / Proteínas do Citoesqueleto / RNA Interferente Pequeno / Proteína Quinase C-épsilon Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2016 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fosforilação / Neoplasias da Mama / Células Tumorais Cultivadas / Transfecção / Regulação para Cima / Western Blotting / Proteínas de Homeodomínio / Proteínas do Citoesqueleto / RNA Interferente Pequeno / Proteína Quinase C-épsilon Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2016 Tipo de documento: Artigo