Distribution characteristics of trimethylamine N-oxide and its association with gut microbiota / 南方医科大学学报
Journal of Southern Medical University
;
(12): 455-460, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-264022
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the distribution of trimethylamine N-oxide (TMAO) in healthy adults with different risk factors and explore its association with gut microbiota.</p><p><b>METHODS</b>We collected fasting blood samples and fresh fecal samples from 181 subjects without atherogenesis in the carotid arteries. Plasma TMAO levels of the subjects were determined using stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS). The fecal DNA was extracted, and the 16S rRNA V4 tags were amplified and sequenced by Illumina HiSeq 2000. The association between TMAO and classical cardiovascular risk factors were analyzed. Gut microbial community structure was analyzed with QIIME, and LEfSe was used to identify the biomarkers.</p><p><b>RESULTS</b>The median (IQR) TMAO level was 2.66 (1.96-4.91) µmol/L in the subjects. TMAO level was significantly correlated with body mass index and operational taxonomic units (OTU). Individuals with high TMAO levels were found to have abundant Clostridiales, Phascolarctobacterium, Oscillibacter, and Alistipes but less abundant Anaerosprobacter.</p><p><b>CONCLUSION</b>Chinese subjects have in general low levels of TMAO. TMAO levels are not significantly correlated with the classical cardiovascular risk factors or the gut microbial structures.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bactérias
/
Sangue
/
RNA Ribossômico 16S
/
Biomarcadores
/
Doenças Cardiovasculares
/
Fatores de Risco
/
Cromatografia Líquida
/
Aterosclerose
/
Espectrometria de Massas em Tandem
/
Microbioma Gastrointestinal
Tipo de estudo:
Estudo de etiologia
/
Estudo prognóstico
/
Fatores de risco
Limite:
Adulto
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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