Effect of jiangu erxian pill on proliferation and cell cycle of human osteoblast / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 129-132, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-264088
ABSTRACT
<p><b>OBJECTIVE</b>To further explore the effect of Jiangu Erxian Pill (JGEXP) on proliferation and cell cycle of human osteoblast on the basis of previous clinical and experimental studies.</p><p><b>METHODS</b>Human primary osteoblast were isolated and cultured. The cell proliferation was tested by 3H-thymine incorporation and methyl thiazolyl tetrazolium (MMT) method and the cell cycle was determined by flow cytometry technique.</p><p><b>RESULTS</b>In the medium and high dosage JGEXP groups, the cell proliferation rate and index, and percentage of diploid synthesis phase (S phase) cells were significantly higher than those in the blank control group (P < 0.01 or P < 0.05), and similar to those in the estrogen group; and the cell apoptosis rate and percentage of G0-G1 stage cells were lower than those in the blank control group (P < 0.01 and P < 0.05).</p><p><b>CONCLUSION</b>JGEXP could effectively promote the cell proliferation and differentiation, and prevent the cell apoptosis of osteoblast in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Distribuição Aleatória
/
Ciclo Celular
/
Divisão Celular
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Apoptose
/
Biologia Celular
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2002
Tipo de documento:
Artigo
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