Effect of lupus recipe on IL-6 and IL-10 secretion of splenic cells in vitro in lupoid mice / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 372-375, 2002.
Article
em Zh
| WPRIM
| ID: wpr-264136
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Lupus Recipe (LR) on IL-6 and IL-10 secretion of splenic cells in vitro in lupoid model mice and on anti-dsDNA antibody.</p><p><b>METHODS</b>Chronic graft-versus-host disease model was used in the experiment. The model mice were divided into four groups, the model group was un-treated and the other three groups treated with LR, prednisone and combined treatment (prednisone + LR) respectively. The serum level of ds-DNA antibody, the ConA induced splenic cell proliferation in mice's splenic cell culture as well as the IL-6, IL-10 level in the supernatant of culture were determined after treatment and compared with those of normal controls.</p><p><b>RESULTS</b>(1) The splenic cell proliferative reaction in the model group splenic cells was obviously higher than that of the normal control (P < 0.05); but that in the three treated groups was different from the control insignificantly (P > 0.05); (2) The serum anti-dsDNA in the model group was higher than that in the normal control, 1.75 +/- 0.25 vs 1.20 +/- 0.21 (P < 0.01), while the difference in comparison of the treated groups with the normal control was insignificant, (P > 0.05); (3) Splenic cell IL-6 and IL-10 secretion in the model group induced by ConA was higher than those in the treated groups and the controls significantly (P < 0.05).</p><p><b>CONCLUSION</b>LR reveals the effect of immunosuppressor, which could inhibit the activation of T- and B-cells, reduce the Th2 cytokine formation and auto-antibody production so as to treat lupus erythematosus effectively.</p>
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Baço
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Medicamentos de Ervas Chinesas
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Células Cultivadas
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Interleucina-6
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Interleucina-10
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Biologia Celular
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Alergia e Imunologia
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Doença Enxerto-Hospedeiro
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Imunossupressores
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2002
Tipo de documento:
Article