Effect of arsenic trioxide on apoptosis of pulmonary eosinophile in asthmatic guinea-pigs / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 292-294, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-264160
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of arsenic trioxide (As2O3) on apoptosis of pulmonary eosinophiles (PE) in asthmatic guinea-pigs.</p><p><b>METHODS</b>Thirty guinea-pigs were divided into 3 groups at random, the control group, the asthmatic group and the As2O3 group. The dosage of As2O3 used was 2 mg/kg. The apoptotic PE were labelled by TdT-mediated dUTP nick end labelling technique, and the PE infiltration and apoptosis were detected quantitatively using computerized image analysis technique.</p><p><b>RESULTS</b>In the control group, the amount of infiltrating PE was 4.4 +/- 2.5 cells/HP and the PE apoptotic index (AI) was 0.42 +/- 0.08%. In the asthmatic group, the amount increased (P < 0.01) and AI decreased significantly (P < 0.01). After the asthmatic animals had been treated with As2O3, the two parameters changed reversedly significantly (P < 0.01), and there was a significantly negative correlation between them (r = -0.949, P < 0.01).</p><p><b>CONCLUSION</b>The PE apoptosis abnormality is one of the important mechanisms that cause bronchial asthma, As2O3 could alleviate the airway inflammation through promoting PE apoptosis and lower PE infiltration. Low dose of As2O3 is proved to be effective with relative safety, it also has potential value in treating asthma.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óxidos
/
Patologia
/
Farmacologia
/
Arsenicais
/
Asma
/
Processamento de Imagem Assistida por Computador
/
Distribuição Aleatória
/
Ovalbumina
/
Apoptose
/
Eosinófilos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2002
Tipo de documento:
Artigo
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