Characterization of phenol biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3 / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 163-172, 2003.
Article
em En
| WPRIM
| ID: wpr-264280
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the characteristic and biochemical mechanism about the phenol biodegradation by bacterial strains ZD 4-1 and ZD 4-3.</p><p><b>METHODS</b>Bacterial strains ZD 4-1 and ZD 4-3 were isolated by using phenol as the sole source of carbon and energy, and identified by 16S rDNA sequence analysis. The concentrations of phenol and total organic carbon (TOC) were monitored to explore the degradation mechanism. The biodegradation intermediates were scanned at 375 nm by using a uv-vis spectrophotometer. The enzyme assays were performed to detect the activities of dioxygenases.</p><p><b>RESULTS</b>Bacterial strains ZD 4-1 and ZD 4-3 were identified as Comamonas testosteroni and Pseudomonas aeruginosa by 16S rDNA sequence analysis, respectively. The growth of the two strains was observed on a variety of aromatic hydrocarbons. The strains ZD 4-1 and ZD 4-3 metabolized phenol via ortho-pathways and meta-pathways, respectively. In addition, the results of enzyme assays showed that the biodegradation efficiency of phenol by meta-pathways was higher than that by ortho-pathways. Finally, the results of induction experiment indicated that the catechol dioxygenases, both catechol 1,2-dioxygenase (C120) and catechol 2,3-dioxygenase (C230), were all inducible.</p><p><b>CONCLUSION</b>The strains ZD 4-1 and ZD 4-3 metabolize phenol through ortho-pathways and meta-pathway, respectively. Furthermore, the biodegradation efficiency of phenol by meta-pathways is higher than that by ortho-pathways.</p>
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1
Índice:
WPRIM
Assunto principal:
Oxigenases
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Farmacologia
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Fisiologia
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Pseudomonas aeruginosa
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Poluentes da Água
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Biodegradação Ambiental
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Fenol
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Comamonas testosteroni
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Desinfetantes
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Metabolismo
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2003
Tipo de documento:
Article