Effect of ionizing radiation on the expression of p16, cyclinD1 and CDK4 in mouse thymocytes and splenocytes / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 47-52, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-264294
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ionizing radiation on the expression of p16, CyclinD1, and CDK4 in mouse thymocytes and splenocytes.</p><p><b>METHODS</b>Fluorescent staining and flow cytometry analysis were employed for the measurement of protein expression.</p><p><b>RESULTS</b>In time course experiments, it was found that the expression of p16 protein was significantly increased at 8, 24, and 48 h for thymocytes (P < 0.05, P < 0.01, and P < 0.05, respectively) and at 24 h for splenocytes (P < 0.05) after whole body irradiation (WBI) with 2.0 Gy X-rays. However, the expression of CDK4 protein was significantly decreased from 8 h to 24 h for thymocytes (P < 0.05-P < 0.01) and from 8 h to 72 h for splenocytes (P < 0.05-P < 0.01). In dose effect experiments, it was found that the expression of p16 protein in thymocytes and splenocytes was significantly increased at 24 h after WBI with 1.0, 2.0, and 4.0 Gy (P < 0.05-P < 0.01), whereas the expression of CDK4 protein was significantly decreased with 2.0 Gy for thymocytes (P < 0.05) and 0.5-6.0 Gy for splenocytes (P < 0.05-P < 0.01). Results also showed that the expression of CyclinD1 protein decreased markedly in both thymocytes and splenocytes after exposure.</p><p><b>CONCLUSION</b>The results indicate that the expression of p16 protein in thymocytes and splenocytes can be induced by ionizing radiation, and the p16-CyclinD1/CDK4 pathway may play an important role for G1 arrest of thymocytes induced by X-rays.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doses de Radiação
/
Efeitos da Radiação
/
Baço
/
Timo
/
Raios X
/
Proteínas Proto-Oncogênicas
/
Quinases Ciclina-Dependentes
/
Ciclina D1
/
Inibidor p16 de Quinase Dependente de Ciclina
/
Biologia Celular
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2003
Tipo de documento:
Artigo
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