Study of increased sensitivity on Tca8113 cell line to cisplatin by nm23-H1 in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 475-478, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-264384
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of sensitivity variation to cisplatin caused by nm23-H1.</p><p><b>METHODS</b>The samles was divided into two groups Tca8113 group and Tca8113/nm23-H1 group. Using MTT and flow cytometer, the changes of cell mortality rate, apoptosis and mitochondrial membrane potential were detected. By VG PQ Excell, the changes of the intracellular platinum were detected.</p><p><b>RESULTS</b>In vitro the cell mortality rate and apoptosis were increased in Tca8113/nm23-H1 group, comparing with Tea8113 group. Mitochondrial membrane potential was decreased in Tca8113/nm23-H1 group. The intracellular platinum was increased significantly in Tca8113/nm23-H1 group. This effect could be inhibited by oubain which was an inhibitor of Na+/K+-ATP.</p><p><b>CONCLUSION</b>nm23-H1 can increase the sensitivity of cisplatin on Tca8113 cell line. The mitochondrial membrane potential was decreased by nm23-H1 so that intracellular platinum was increased and finally increased the apoptosis or necrosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas In Vitro
/
Transfecção
/
Linhagem Celular
/
Cisplatino
/
Apoptose
/
Linhagem Celular Tumoral
/
Nucleosídeo NM23 Difosfato Quinases
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2008
Tipo de documento:
Artigo
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