Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 312-319, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-264430
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma (SACC-2) cells in vitro.</p><p><b>METHODS</b>To detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase (Caspase 3 and Caspase 8) and proliferating cell nuclear antigen (PCNA) in SACC-2 cells, immunohistochemistry staining was used.</p><p><b>RESULTS</b>According the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was (4.99+/-0.23) microg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant (P<0.01). The expression of PCNA was reverse (P<0.01).</p><p><b>CONCLUSION</b>Salidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenóis
/
Técnicas In Vitro
/
Neoplasias das Glândulas Salivares
/
Imuno-Histoquímica
/
Apoptose
/
Carcinoma Adenoide Cístico
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Glucosídeos
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2008
Tipo de documento:
Artigo
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