Combination of Loop-Mediated Isothermal Amplification Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 312-315, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-264582
ABSTRACT
A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the fla gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.l. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.l. in human serum samples.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA Bacteriano
/
Doença de Lyme
/
Grupo Borrelia Burgdorferi
/
China
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Técnicas de Amplificação de Ácido Nucleico
/
Diagnóstico
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2015
Tipo de documento:
Artigo
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