Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 293-299, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-264710
ABSTRACT
Objective A calcium-activated chloride current (IClCa) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have IClCa current inducing properties. Results We showed the detection of transcripts representing several members of these families, i.e. chloride channel calcium-activated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced IClCa in DRG neurons.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Nervo Isquiático
/
Expressão Gênica
/
Primers do DNA
/
Canais de Cloreto
/
Axotomia
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Gânglios Espinais
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Inglês
Revista:
Neuroscience Bulletin
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS