Expression of core-binding factor a1 by human skin fibroblasts induced in vitro / 中华外科杂志
Chinese Journal of Surgery
;
(12): 592-595, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-264766
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.</p><p><b>METHODS</b>The fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.</p><p><b>RESULTS</b>TNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.</p><p><b>CONCLUSION</b>Human skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Pele
/
Fatores de Transcrição
/
RNA Mensageiro
/
Osteocalcina
/
Células Cultivadas
/
Fator de Crescimento Transformador beta
/
Colágeno
/
Fator de Necrose Tumoral alfa
/
Proteínas Serina-Treonina Quinases
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2002
Tipo de documento:
Artigo
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