Study on isozymes in six species of Curcuma / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore genetic relationships of the 39 materials in six species of Curcuma.</p><p><b>METHOD</b>The peroxidase isozyme (POD) and esterase isozyme (EST) were studied using vertical slab polyacrylamide gel electrophoresis (PAGE) technique, and the zymograms were analyzed using the software of NTSYSpc2. 1.</p><p><b>RESULT</b>The interspecific zymogramatic differences were obvious. Each species possessed its own specific zymogram distinguishing form the others. In the analysis of EST isozyme, C. phaeocaulis, C. wenyujin, C. kwangsiensis and C. chuanhuangjiang had their own specific zymogram. In the analysis of POD isozyme, just C. phaeocaulis and C. kwangsiensis had their specific zymogram.</p><p><b>CONCLUSION</b>The genetic relationships are not associated with the geographical distributions and the genetic relationship between C. longa and C. sichuanensis are very close.</p>