TNF-α stimulates bone marrow mesenchymal stem cell VCAM-1 production by ERK signaling pathway / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1568-1571, 2013.
Article
em Zh
| WPRIM
| ID: wpr-264974
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to explore the effect of TNF-α on the vascular cell adhesion molecule 1 (VCAM-1) expression of human bone marrow mesenchymal stem cells (BMMSC) and the relationship between this process and ERK signalling pathway. BMMSC were isolated by density gradient centrifugation combined with adherent culture method, and then identified by surface antigen expression and differentiation potential. Flow cytometry was used to detect expression of VCAM-1 on BMMSC exposed to TNF-α at different concentrations, and the effect of ERK inhibitor U0126 on VCAM-1 of BMMSC. ERK signaling pathway activation was analyzed by Western blot. The results showed that BMMSC positively expressed CD29, CD69, CD44, CD105, and negatively expressed CD34, CD45. BMMSC could be induced to differentiate into osteoblasts and adipocytes. Flow cytometry analysis showed that after the TNF-α stimulation, the expression of VCAM-1 on BMMSC increased in a dose-dependent manner. And this increase was inhibited by U0126. TNF-α caused activation of ERK signal pathway, and U0126 suppressed this effect induced by TNF-α. It is concluded that TNF-α can increase expression of VCAM-1 of BMMSC via ERK signaling pathway.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Células da Medula Óssea
/
Células Cultivadas
/
Fator de Necrose Tumoral alfa
/
Molécula 1 de Adesão de Célula Vascular
/
Sistema de Sinalização das MAP Quinases
/
Biologia Celular
/
Células-Tronco Mesenquimais
/
Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2013
Tipo de documento:
Article