Effect of paraoxon and chlorpyrifos on the nicotinic autoreceptor function in rat cortical synaptosomes / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 188-190, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-265081
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of organophosphorus insecticides (OPs) on the nicotinic autoreceptor function (NAF) in rat cortical synaptosomes and to understand alternative target of the OPs for human and other animals.</p><p><b>METHODS</b>In vitro experiment, synaptosomes from the rats were incubated with [(3)H] choline and then superfused with physiological buffer. The [(3)H] acetylcholine release from the synaptosomes after the addition of paraoxon or chlorpyrifos to the superfusion system was recorded and the changes of NAF were calculated. In vivo experiment, NAF and acetylcholinesterase (AChE) activity in the cortical synaptosomes in the adult rats dosed with chlorpyrifos were also determined 96 h after OPs treatment.</p><p><b>RESULTS</b>Paraoxon caused slight effect on NAF (average inhibition rate < 23%) while chlorpyrifos oxon caused > 100% of inhibition on NAF in vitro. Chlorpyrifos markedly reduced NAF by 66% 96 h after treatment and inhibited the AChE activity by 91% in vivo.</p><p><b>CONCLUSION</b>The OPs may have different effects on the NAF of rat cortical synaptosomes while chlorpyrifos may certainly inhibit the NAF in cortical synaptosomes of adult rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Paraoxon
/
Fisiologia
/
Sinaptossomos
/
Técnicas In Vitro
/
Encéfalo
/
Acetilcolina
/
Receptores Nicotínicos
/
Secreções Corporais
/
Clorpirifos
/
Toxicidade
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS