Preliminary analysis of mutations in exon 5 of IkappaBalpha gene in Hodgkin lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 341-344, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-265108
ABSTRACT
<p><b>OBJECTIVE</b>To study the mutations in exon 5 of IkappaBalpha gene and their significance in Hodgkin lymphoma.</p><p><b>METHODS</b>CD30-positive Reed-Sternberg cells and surrounding reactive lymphocytes were selected by laser microdissection technology from 6 microm-thick histologic sections of 7 Hodgkin lymphoma cells studied. Genomic DNA of these cells was extracted according to Qiagen micro DNAkit guide. Semi-nested polymerase chain reaction (PCR) and direct DNA sequencing were used to analyze exon 5 of IkappaBalpha gene.</p><p><b>RESULTS</b>A mutation in exon 5 was identified in 3 of the 7 Hodgkin lymphoma cases. No mutation found in the surrounding reactive lymphocytes. The mutation site was located at codon 2084 (T-A, NT_026437), resulting in formation of a stop codon.</p><p><b>CONCLUSION</b>The mutation identified in exon 5 of the IkappaBalpha gene in Hodgkin lymphoma may play a role in the pathogenesis of Hodgkin lymphoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA de Neoplasias
/
Doença de Hodgkin
/
Análise Mutacional de DNA
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Éxons
/
Mutação Puntual
/
Deleção de Genes
/
Proteínas I-kappa B
/
Inibidor de NF-kappaB alfa
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Artigo
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