Changes of fibronectin and type IV collagen expression in cultured rat mesangial cells transfected with Smad 2 vector / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 97-100, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-265185
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of fibronectin (FN) and type IV collagen (ColIV) expression in cultured rat mesangial cells (MsC) transfected with Smad 2 vector and to investigate the molecular mechanism of glomerular extracellular matrix accumulation in glomerulosclerosis via transforming growth factor-beta (TGF-beta)/Smad signal pathway.</p><p><b>METHODS</b>Smad 2 vector was transfected into MsC by calcium phosphate. Western blot analysis was used to detect Smad 2 protein. The expression of FN and ColIV proteins and their mRNAs was determined by Western blot and reverse transcriptase-polymerase chain reaction respectively.</p><p><b>RESULTS</b>Four MsC clones (T-12, T-31, T-35, T-40) with Smad 2 overexpression were established. The expression of FN and ColIV was significantly increased at mRNA and protein levels in two (T-12, T-31). Compared with controls, the expression of FN proteins and mRNAs in these two clones was 2.4 times (P < 0.05) and 2.7 times (P < 0.05) higher respectively. The expression of ColIV proteins and mRNAs was 2.9 times (P < 0.01) and 3.3 times (P < 0.01) higher respectively.</p><p><b>CONCLUSIONS</b>It is postulated that Smad 2 in TGF-beta/Smad signal pathway is important in promoting the accumulation of FN and ColIV in sclerotic glomeruli of diseased kidneys.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
RNA Mensageiro
/
Transfecção
/
Transdução de Sinais
/
Células Cultivadas
/
Fator de Crescimento Transformador beta
/
Fibronectinas
/
Biologia Celular
/
Colágeno Tipo IV
/
Células Mesangiais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Artigo
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