Study on HPLC fingerprint of Trachelospermum jasminoides / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 727-730, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-265318
ABSTRACT
<p><b>OBJECTIVE</b>To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.</p><p><b>METHOD</b>The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm.</p><p><b>RESULT</b>The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.</p><p><b>CONCLUSION</b>The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Padrões de Referência
/
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Apocynaceae
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Artigo
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