HPLC-CAD in optimizating of saponins extraction from Radix et Rhizoma Notoginseng / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an optimized method for extracting saponins from Radix et Rhizoma Notoginseng (Sanqi).</p><p><b>METHOD</b>HPLC coupled with charged aerosol detector (CAD) was used for saponins determination. Peak areas of the main saponins notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rg2, Rh1 and Rd in Sanqi were monitored to evaluate the extract effectiveness. One-factor analysis was conducted to investigate the factors of particle size, solvent, extraction method and soaking time. Orthogonal experiment design L9 (3(4)) with different level of concentration of solvents, extraction time, extraction times and volume of the solvent, was implemented to determine the optimized extract condition.</p><p><b>RESULT</b>The optimum extract technique was that the dried and well pulverized (passed through a 80 mesh (180 +/-7.6) microm sample 1.0 g was extracted in 20 mL methanol by reflux (1.5 h) after soaked at room temperature for 0.5 h.</p><p><b>CONCLUSION</b>The optimized technique is simple and high extraction efficiency. It provide basic data for further study on Sanqi.</p>