Effect of human serum extracted by two different methods on human dermal fibroblast growth in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the optimal method for extracting human serum that retains rich growth factors.</p><p><b>METHODS</b>Human serum was isolated by centrifugation of coagulated whole blood or by Anitua's method, and the concentrations of PDGF-AB and transforming growth factor-β1 (TGF-β1) in the serum samples were measured. Human dermal fibroblasts were cultured in the presence of 10% feral bovine serum or 10% human serum obtained, and the cell morphology, viability and proliferative activity of the cells were evaluated.</p><p><b>RESULTS</b>The fibroblasts grew well in all the media with good viability. The cells cultured in the presence of human serum isolated by centrifugation of coagulated whole blood, which had the richest content of growth factors, showed the greatest cell number and cell viability among the groups (P<0.05), a result consistent with the growth curve and MTT curve.</p><p><b>CONCLUSION</b>Centrifugation of coagulated whole blood retains high contents of growth factors in human serum to better promote cell growth, and is simple, cost-effective and most efficient for serum isolation.</p>