Endothelial dysfunction induced by high glucose is associated with decreased ATP-binding cassette transporter G1 expression / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of ATP-binding cassette transporter G1 (ABCG1) in endothelial dysfunction induced by high glucose.</p><p><b>METHODS</b>Human aortic endothelial cells (HAECs) were incubated in the presence of 5.6 or 30 mmol/L glucose for 24-72 h with or without a 2-h pretreatment with the LXR agonist 22(R)-hydroxycholesterol. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCG1; the intracellular cholesterol efflux and endothelial nitric oxide synthase (eNOS) activity were measured by scintillation counting.</p><p><b>RESULTS</b>High glucose time-dependently suppressed ABCG1 expression and cholesterol efflux to HDL in HAECs. High glucose also decreased eNOS activity. ABCG1 down-regulation induced by high glucose, along with decreased cholesterol efflux and eNOS activity, was abolished by treatment of the cells with the LXR agonist.</p><p><b>CONCLUSION</b>Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression.</p>