Effect of gemcitabine in enhancing the radiosensitivity of HepG2 hepatoma cells and the possible mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1993-1996, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-265733
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of gemcitabine in enhancing the radiosensitivity of hepatoma cell line HepG2 and explore its mechanisms.</p><p><b>METHODS</b>Clonogenic survival assay is employed to calculate the ratios of L-Q model radiation biology parameters and radiosensitization after different doses of irradiation. Flow cytometry was used to detect the changes in HepG2 cell cycle and apoptosis rate after gemcitabine treatment and radiation exposure.</p><p><b>RESULTS</b>The survival fraction at 2 Gy of HepG2 cells treated with gemcitabine was significantly lower, and the value of alpha was significantly higher than those of untreated cells. GEM treatment increased the percentage of radiation-induced G0/G1 phase cells and cell apoptosis.</p><p><b>CONCLUSION</b>Gemcitabine can significantly enhance the radiosensitivity of HepG2 cells by enhancing radiation-induced cell cycle arrest in G0/G1 phase and cell apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Radiossensibilizantes
/
Tolerância a Radiação
/
Apoptose
/
Desoxicitidina
/
Células Hep G2
/
Pontos de Checagem do Ciclo Celular
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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