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Effect and related mechanism of seaweed polysaccharide on apoptosis of fibroblast-like synoviocytes in patients with rheumatoid arthritis / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 961-966, 2011.
Artigo em Chinês | WPRIM | ID: wpr-265777
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and related mechanism of seaweed polysaccharide (SP) on apoptosis of fibroblast-like synoviocytes in patients with rheumatoid arthritis (RA-FLS).</p><p><b>METHODS</b>RA-FLS were in vitro cultured using modified tissue culture method. Effect of SP (0, 15, 20, and 25 mg/mL, respectively) at different time points (0, 3, 4, and 5 days, respectively) on the proliferation and apoptosis of RA-FLS, and protein expressions of Caspase-3, Bax, and Bcl-2 was detected by cell counting kit-8 (CCK-8) assay, Hoechst 33258 staining assay, TUNEL assay, and Western blot, respectively.</p><p><b>RESULTS</b>Compared with 0 mg/mL SP at the same time point, the proliferation of RA-FLS was inhibited, and the apoptosis was promoted 3, 4, and 5 days after intervened by 15, 20, and 25 mg/mL SP, respectively (P<0.01) in time- and dose-dependent manners. RA-FLS Bax protein expression was up-regulated, Bcl-2 protein expression down-regulated, Caspase-3 activated and split by 15, 20, and 25 mg/mL SP, respectively for 4 days (P<0.05, P<0.01). Besides, the changes were in a dose-dependent manner.</p><p><b>CONCLUSIONS</b>SP could inhibit RA-FLS proliferation and induce its apoptosis in dose- and time-dependent manners. Its apoptosis mechanism might be through up-regulating intracellular Bax protein expression and down-regulating Bcl-2 protein expression, thus influencing the mitochondrion signaling pathway, further promoting Caspase-3 activation and split, resulting in the apoptosis of RA-FLS.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Polissacarídeos / Artrite Reumatoide / Alga Marinha / Membrana Sinovial / Células Cultivadas / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Biologia Celular Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Polissacarídeos / Artrite Reumatoide / Alga Marinha / Membrana Sinovial / Células Cultivadas / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Biologia Celular Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2011 Tipo de documento: Artigo