Estradiol stimulates proliferation of prostatic smooth muscle cells via estrogen receptor alpha and IGF1 / 中华男科学杂志
National Journal of Andrology
;
(12): 131-135, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-266200
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of estrogen receptor alpha (ERa) and insulin-like growth factor 1 (IGF1) on the proliferation of prostatic smooth muscle cells (PSMCs) in vitro.</p><p><b>METHODS</b>The ERalpha shRNA expression frame was subcloned to the pGSadeno adenovirus vector by homologous recombination technology to construct the pGSaaeno-ERalpha vector. After the mouse PSMCs were transfected in vitro by pGSaaeno-ERalpha, the mRNA and protein expression levels of ERalpha were detected by RT-PCR and Western blot respectively. The expression of IGF1 in the ERa-reduced cells was determined by Western blot 6 hours after treatment with 17beta-estradiol (E2) at 10(-8) mol/L. The post-transfection activity of estrogen or exogenous IGF1 in the proliferation of PSMCs was evaluated by MTT chlormetric analysis.</p><p><b>RESULTS</b>After treatment with E2, the proliferation of PSMCs and the expression of the IGF1 gene were significantly increased in the normal control group (P <0.05), but not obviously changed in the ERalpha-siRNA group (P> 0.05). And exogenous IGF1 failed to induce the proliferation of the ERalpha-reduced PSMCs.</p><p><b>CONCLUSION</b>E2 induces the expression of IGF1 via ERalpha, and IGFl, with the interaction of ERalpha, promotes the proliferation of PSMCs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Próstata
/
RNA Mensageiro
/
Fator de Crescimento Insulin-Like I
/
Células Cultivadas
/
Biologia Celular
/
Miócitos de Músculo Liso
/
Receptor alfa de Estrogênio
/
Proliferação de Células
/
Estradiol
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2011
Tipo de documento:
Artigo
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