Significance of IL-1beta-induced ectopic expression of CDX2 in the intestinal metaplasia of gastric epithelium / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 524-527, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-266315
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of IL-1beta on the expression of CDX2 in human gastric epithelial cell line GES-1 and its role in the intestinal metaplasia.</p><p><b>METHODS</b>GES-1 cells were treated with IL-1beta in different concentrations and the expressions of CDX2 mRNA and protein were detected by real-time PCR, immunocytochemistry and Western blot at different time points. GES-1 cells were then pre-treated with NF-KappaB pathway inhibitor PDTC, and the expression of CDX2 mRNA and protein induced by IL-1beta were detected. The cell ultra-structure of GES-1 cells was observed by electronic microscope after GES-1 being treated with IL-1beta for 25 days.</p><p><b>RESULTS</b>Levels of CDX2 mRNA and protein were 0.0749 + or - 0.0021 and 0.56 + or - 0.04 in the cells treated with 1 microg/L IL-1beta(P<0.05). After pre-treatment with PDTC, levels of CDX2 mRNA and protein were 0.0006 + or - 0.0002 and 0.40 + or - 0.06(P<0.05). Some changes in the cell ultra-structure of GES-1 were found by electronic microscope when GES-1 was treated with IL-1beta for 25 days.</p><p><b>CONCLUSION</b>IL-1beta can stimulate CDX2 mRNA and protein expression in GES-1 cells through the NF-KappaB signal pathway, indicating that IL-1beta plays an important role in the intestinal metaplasia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
RNA Mensageiro
/
Linhagem Celular
/
Proteínas de Homeodomínio
/
Biologia Celular
/
Epitélio
/
Interleucina-1beta
/
Fator de Transcrição CDX2
/
Mucosa Gástrica
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo
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