Expression of MDR1 and KIT in imatinib-resistant gastrointestinal stromal tumor cells / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 506-509, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-266319
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between imatinib resistance and genes MDR1 and KIT in gastrointestinal stromal tumor (GIST) cells.</p><p><b>METHODS</b>The MDR1 and KIT mRNA level in GIST882-R and GIST882-S cells were detected by RT-PCR. Immunocytochemistry and Western blot were employed to detect P-gp and CD117 expression in GIST882-R and GIST882-S cells.</p><p><b>RESULTS</b>The relative expression of MDR1 mRNA was 0.321 + or - 0.033 in GIST882-R and 0.157 + or - 0.056 in GIST882-S cells, and the difference was statistically significant (P<0.05). The relative expression of KIT mRNA was 0.389 + or - 0.063 in GIST882-R and 0.339 + or - 0.067 in GIST882-S, and the difference was not statistically significant (P>0.05). The relative density of P-gp was 0.443 + or - 0.058 in GIST882-R and 0.237 + or - 0.094 in GIST882-S, and the difference was statistically significant (P<0.05). The relative density of CD117 was 0.744 + or - 0.123 in GIST882-R and 0.704 + or - 0.094 in GIST882-S, and the difference was not statistically significant (P>0.05).</p><p><b>CONCLUSIONS</b>Over-expression of gene MDR1 may be associated with imatinib resistance in GIST. KIT may not be involved in imatinib resistance.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Piperazinas
/
Pirimidinas
/
Benzamidas
/
Moléculas de Adesão Celular
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Proteínas Proto-Oncogênicas c-kit
/
Resistencia a Medicamentos Antineoplásicos
/
Linhagem Celular Tumoral
/
Tumores do Estroma Gastrointestinal
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo
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