Protective effect of 3,4-oxo-isopropylidene-shikimic acid on vascular endothelial cell injured by hydrogen peroxide / 药学学报
Acta Pharmaceutica Sinica
;
(12): 897-899, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-266558
ABSTRACT
<p><b>AIM</b>To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on H2O2 (200 mol.L-1, 4 h) injured human umbilical vein endothelial cells (HUVEC).</p><p><b>METHODS</b>Morphological change was observed under microscop. Cell viability was assessed by MTT assay. The release of intracellular lactate dehydrogenase (LDH) and NO was assessed by colorimetry. Radioimmunoassay was used to assess 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha).</p><p><b>RESULTS</b>Pretreatment with ISA for 6 h alleviated the morphological damage of H2O2 induced HUVECs. At the concentration of 1-100 mumol.L-1, ISA prevented the inhibitory effect on cell viability induced by H2O2 in dose-dependent manner, but increased the ratio of cell viability from 60.4% to 84.3%. ISA reduced LDH release and increased the level of NO and 6-keto-PGF1 alpha in H2O2 induced HUVECs.</p><p><b>CONCLUSION</b>ISA exerted protective effect on H2O2 injured HUVEC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Ácido Chiquímico
/
Veias Umbilicais
/
6-Cetoprostaglandina F1 alfa
/
Sobrevivência Celular
/
Células Cultivadas
/
Substâncias Protetoras
/
Biologia Celular
/
Células Endoteliais
Limite:
Humanos
/
Recém-Nascido
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS