Effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by hypoxia / 药学学报
Acta Pharmaceutica Sinica
; (12): 739-742, 2003.
Article
em Zh
| WPRIM
| ID: wpr-266613
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by hypoxia in vitro.</p><p><b>METHODS</b>The cultured bovine cerebromicrovascular endothelial cells were exposed to NaCN in glucose-free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) and flow cytometry. The expression of caspase-3 was detected by immunocytochemical method. Four caspase inhibitors were used to validate the effect of caspases on cell apoptosis.</p><p><b>RESULTS</b>NaCN in glucose-free medium initiated cerebromicrovascular endothelial cell injury markedly and typical apoptotic cells were found in this model. The expression of caspase-3 increased significantly. Four caspase inhibitors decreased the number of injured cells. Selective inhibitor of caspase-1 and -6 reduced expression of caspase-3 significantly.</p><p><b>CONCLUSION</b>The results suggest that caspases family plays an important role in cerebromicrovascular endothelial cell apoptosis induced by NaCN and caspase-3 acts on the downstream of caspase-1 and -6 in protease cascade action to induce apoptosis.</p>
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Índice:
WPRIM
Assunto principal:
Oligopeptídeos
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Farmacologia
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Cianeto de Sódio
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Encéfalo
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Hipóxia Celular
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Células Cultivadas
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Apoptose
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Caspases
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Biologia Celular
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Células Endoteliais
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2003
Tipo de documento:
Article