Determination of schisandrin in shengmaiyin by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 39-72, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-266823
ABSTRACT
<p><b>OBJECTIVE</b>To determine the concentration of schisandrin in Shengmaiyin with HPLC.</p><p><b>METHOD</b>The sample was extracted with ethyl acetate through supersonic wave. The solution was filtrated and evaporated. The residue was resolved with methanol and determined by HPLC using PHENOMENEX C18 (4.6 mm x 250 mm, 5 microns) chromatographic column, methanol-acetonitrile-water (151510) as mobile phase. The wavelength for detection was 254 nm.</p><p><b>RESULT</b>The peak of schizandrin appears on about 7.10 minutes. The standard curves of schizandrin were linear in the concentration range of 0.2-2.0 micrograms, r = 0.9996. The average recovery of schizandrin were 100.5% (RSD 2.84%).</p><p><b>CONCLUSION</b>This method was found to be sensitive, quick and accurate for the measurement of schizandrin concentrations in Shengmaiyin.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Compostos Policíclicos
/
Medicamentos de Ervas Chinesas
/
Química
/
Administração Oral
/
Cromatografia Líquida de Alta Pressão
/
Lignanas
/
Schisandra
/
Ciclo-Octanos
/
Combinação de Medicamentos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2003
Tipo de documento:
Artigo
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