Recombinant Human Erythropoietin Augments Neovascularization Responses in a Neonatal Rat Model of Premature Brain Damage by Phosphatidylinositol 3 Kinase/Akt Pathway / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 854-858, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-266898
ABSTRACT
<p><b>BACKGROUND</b>Recombinant human-erythropoietin (rh-EPO) has therapeutic efficacy for premature infants with brain damage during the active rehabilitation and anti-inflammation. In the present study, we found that the rh-EPO was related to the promotion of neovascularization. Our aim was to investigate whether rh-EPO augments neovascularization in the neonatal rat model of premature brain damage through the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway.</p><p><b>METHODS</b>Postnatal day 5 (PD5), rats underwent permanent ligation of the right common carotid artery and were exposed to hypoxia for 2 h. All the rat pups were randomized into five groups as follows (1) control group; (2) hypoxia-ischemic (HI) group; (3) HI + LY294002 group; (4) HI + rh-EPO group; and (5) HI + rh-EPO + LY294002 group. The phospho-Akt protein was tested 90 min after the whole operation, and CD34, vascular endothelial growth factor receptor 2 (VEGFR2), and vascular endothelial growth factor (VEGF) were also tested 2 days after the whole operation.</p><p><b>RESULTS</b>In the hypoxic and ischemic zone of the premature rat brain, the rh-EPO induced CD34+ cells to immigrate to the HI brain zone (P < 0.05) and also upregulated the VEGFR2 protein expression (P < 0.05) and VEGF mRNA level (P < 0.05) through the PI3K/Akt (P < 0.05) signaling pathway when compared with other groups.</p><p><b>CONCLUSIONS</b>The rh-EPO treatment augments neovascularization responses in the neonatal rat model of premature brain damage through the PI3K/Akt signaling pathway. Besides, the endogenous EPO may exist in the HI zone of rat brain and also has neovascularization function through the PI3K/Akt signaling pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Encéfalo
/
Proteínas Recombinantes
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Transdução de Sinais
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Eritropoetina
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Ratos Sprague-Dawley
/
Neovascularização Fisiológica
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Antígenos CD34
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Hipóxia-Isquemia Encefálica
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Receptor 2 de Fatores de Crescimento do Endotélio Vascular
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2017
Tipo de documento:
Artigo
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