Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis).</p><p><b>METHOD</b>Applying the inter-simple sequence repeats (ISSR) markers technology.</p><p><b>RESULT</b>Twenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS (genetic similarity) value was 0.6675-0.8210. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship.</p><p><b>CONCLUSION</b>It is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study.</p>