Raman spectroscopic study on effect of danshen injection on human erythrocyte membranes and its mechanism / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>Danshen, as a traditional Chinese medicine for promoting blood circulation and removing blood stasis, is widely applied in improving human erythrocyte deformability in clinics. But its direct effect on erythrocyte membranes is still unclear.</p><p><b>METHOD</b>In this essay, the confocal Raman technique was adopted to measure the changes in Raman spectra of human erythrocytes before and after the administration of Danshen injection.</p><p><b>RESULT</b>The results showed slight changes in group conformations corresponding to erythrocyte membranes after the administration of danshen injection. Specifically, 1064, 1126 cm(-1) spectral lines attributed to phospholipid molecule acyl C-C skeleton anti-conformation were obviously weakened, whereas 1091 cm(-1) spectral line attributed to phospholipid molecule acyl C-C skeleton guache conformation notably intensified. Besides, the longitudinal order-parameter in chains (Strans) of phospholipids was reduced significantly.</p><p><b>CONCLUSION</b>Danshen injection can transfer erythrocyte membrane phospholipid molecule acyl C-C skeleton anti-conformation to guache conformation, indicating the increase in liquidity of erythrocyte membrane phospholipids and erythrocytes. Danshen injection's effect in improving erythrocyte membrane structure and function may be the intrinsic mechanism for its pharmacological effect as a traditional Chinese medicine.</p>