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Effects of modified Sanhuang decoction () enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats / 中国结合医学杂志
Chinese journal of integrative medicine ; (12): 865-869, 2014.
Artigo em Inglês | WPRIM | ID: wpr-267201
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Modified Sanhuang Decoction (, MSD) enema on the serum tumor necrosis factor alpha (TNF-α) and colonic mucosa interleukin-1β (IL-1β), interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats.</p><p><b>METHODS</b>Forty-five male Wistar rats were randomly divided into 4 groups normal group (n=12), model group (n=11), salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline, while in the SASP group and MSD group were clystered with SASP and MSD enema, respectively. After drug administration (10 mL/kg body weight, for 7 days), colonic gross changes and colonic mucosa histology were observed, serum TNF-α and colonic mucosa IL-1β, IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay, respectively.</p><p><b>RESULTS</b>As compared with the normal group, the experimental UC rats, the colonic mucosal damage index scores (CMDIs), histopathological scores (HS) and the serum TNF-α and colonic mucosa IL-1β, IL-6 levels significantly increased (P<0.05 or P<0.01). In the MSD and SASP groups, the ulcer area significantly reduced, and edema disappeared. The CMDIs, HS, the serum TNF-α and colonic mucosa IL-1β, IL-6 levels in the MSD and SASP groups significantly decreased (P<0.05 or P<0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P<0.05), but there were no significant differences in HS, serum TNF-α or colonic mucosa IL-1β, IL-6 levels between the MSD and SASP groups.</p><p><b>CONCLUSION</b>MSD enema can improve colonic mucosa impairment and decrease serum TNF-α and colonic mucosa IL-1β, IL-6 levels in experimental UC.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Terapêutica / Sangue / Medicamentos de Ervas Chinesas / Colite Ulcerativa / Interleucina-6 / Ratos Wistar / Colo / Enema / Interleucina-1beta / Mucosa Intestinal Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese journal of integrative medicine Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Terapêutica / Sangue / Medicamentos de Ervas Chinesas / Colite Ulcerativa / Interleucina-6 / Ratos Wistar / Colo / Enema / Interleucina-1beta / Mucosa Intestinal Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese journal of integrative medicine Ano de publicação: 2014 Tipo de documento: Artigo