miR-124 suppresses cell proliferation and invasion in gastric carcinoma and its mechanism / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 497-500, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-267513
ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanism of miR-124 suppressing the proliferation and invasion of gastric cancer cells.</p><p><b>METHODS</b>SPHK1 3'UTR-luciferase vector was constructed and luciferase reporter gene assay was employed to examine the effect of miR-124 on luciferase activity. Human gastric cancer MGC-803 cells were transfected with miR-124 mimics, and then Western blot was performed to detect the expression of SPHK1 protein.</p><p><b>RESULTS</b>Luciferase reporter vector system confirmed that SPHK1 was a target gene of miR-124. Western blot showed that the expression of SPHK1 protein was inhibited by miR-124. After transfection of miR-124 mimics or SPHK1 siRNA for 12 h, 24 h and 48 h, respectively, MTT assay showed that the A values of the three groups were significantly different (P < 0.05), and it was in a time-dependent manner. After transfection of miR-124 mimics or SPHK1 siRNA for 24 h, transwell invasion assay showed that the number of transmembrane cells was 54.6 ± 8.3 in the SPHK1 siRNA group and 47.8 ± 6.6 in the miR-124 mimics group, both were significantly lower than 100.6 ± 11.3 of the control group (P < 0.05), indicating that SPHK1 siRNA can slow down the invasion of MGC-803 cells.</p><p><b>CONCLUSION</b>miR-124 can suppress the cell proliferation and invasion by targeting SPHK1 in gastric carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias Gástricas
/
Proteínas Recombinantes
/
Transfecção
/
Fosfotransferases (Aceptor do Grupo Álcool)
/
MicroRNAs
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS