Your browser doesn't support javascript.
loading
Analysis of larval excretory-secretory antigen and its immunodiagnosis of Angiostrongyliasis cantonensis infection / 南方医科大学学报
Journal of Southern Medical University ; (12): 477-481, 2012.
Artigo em Chinês | WPRIM | ID: wpr-267572
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the diagnostic value of larval excretory-secretory antigen in Angiostrongylus cantonensis (LESA) infection.</p><p><b>METHODS</b>A.cantonensis larvae harvested from mice brain were cultured in vitro. The LESA and the adult worm antigens of A.cantonensis (AWA) were collected and analyzed using SDS-PAGE and Western blotting. Two ELISA systems were established using the two antigens (LESA-ELISA and AWA-ELISA) to detect the serum spectra from different sources.</p><p><b>RESULTS</b>SDS-PAGE and Western blotting displayed fewer protein and antigen bands for LESA than for the adult antigen. Two distinct bands of LESA (with relative molecular masses of 40 000 and 26 000) showed reactivity with the sera from patients with A. cantonensis infection. The serum levels of IgG and IgM antibodies to LESA increased at the beginning of infection in mice, reaching the peak on day 5 after infection and decreased on day 10. Compared with AWA-ELISA, LESA-ELISA showed a lower seropositive ratio in suspected patients with A.cantonensis, with also a lower cross-positive ratio in patients with schistosomiasis and clonorchis sinensis.</p><p><b>CONCLUSION</b>LESA possesses fewer antigen reaction bands than AWA. Although with a slightly lower positive ratio than AWA, LESA has a higher specificity for detecting serum antibodies in suspected cases of A.cantonensis infection, and therefore shows a potential for the diagnosis of angiostrongyliasis especially in the early stage and in current infection.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parasitologia / Ensaio de Imunoadsorção Enzimática / Infecções por Strongylida / Ratos Sprague-Dawley / Angiostrongylus cantonensis / Diagnóstico / Alergia e Imunologia / Larva / Camundongos Endogâmicos BALB C / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2012 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parasitologia / Ensaio de Imunoadsorção Enzimática / Infecções por Strongylida / Ratos Sprague-Dawley / Angiostrongylus cantonensis / Diagnóstico / Alergia e Imunologia / Larva / Camundongos Endogâmicos BALB C / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2012 Tipo de documento: Artigo