Iodoacetamide-induced aquaporin 1 expression in fibroblasts is energy-dependent / 南方医科大学学报
Journal of Southern Medical University
; (12): 28-32, 2011.
Article
em Zh
| WPRIM
| ID: wpr-267679
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).</p><p><b>METHODS</b>Balb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h.</p><p><b>RESULTS</b>IA induced the expression of AQP1 at 4 and 6 h accompanied with cell death. Reverse transcription PCR showed an increased expression of AQP1 mRNA in the cells. AQP1 expression was also upregulated by the inhibitor of microtubule and cytochrome C oxidase.</p><p><b>CONCLUSION</b>A pretranslational regulation occurs in IA-induced AQP1 expression in mouse fibroblasts, and the up-regulated AQP1 accumulation is characterized by mitochondria-related energy dependence.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Regulação para Cima
/
Células Cultivadas
/
Biologia Celular
/
Metabolismo Energético
/
Aquaporina 1
/
Fibroblastos
/
Genética
/
Iodoacetamida
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Article