Effect of asymmetric dimethylarginine on MIF expression and TNF-α and IL-8 secretion in THP-1 monocytes-derived macrophages / 南方医科大学学报
Journal of Southern Medical University
; (12): 1-4, 2011.
Article
em En
| WPRIM
| ID: wpr-267684
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ADMA on macrophage migration inhibitory factor (MIF) expression and tumor necrosis factor-α (TNF-α) and IL-8 secretion in THP-1 monocyte-derived macrophages. METHIDS: THP-1 monocytes were induced to differentiate into macrophages by a 24-h incubation with 160 nmol/L PMA. The THP-1 monocyte-derived macrophages were exposed to different concentrations of ADMA for 24 h, and the changes in MIF mRNA and protein expressions were analyzed with RT-PCR and Western blotting, respectively. Enzyme-linked immunosorbent assay was used to detect the levels of TNF-α and IL-8 in the supernatant of THP-1-derived macrophages following ADMA treatments.</p><p><b>RESULTS</b>ADMA obviously up-regulated MIF mRNA and protein expressions in THP-1-derived macrophages in a concentration- dependent manner. Exposure of the cells to 15 µmol/L ADMA for 24 h showed the most potent effect in up-regulating MIF mRNA and protein expressions. ADMA treatment also resulted in a dose-dependent increase of the levels of TNF-α and IL-8 in the culture supernatant of the macrophages, and the peak levels occurred following the treatment with 15 µmol/L ADMA.</p><p><b>CONCLUSION</b>ADMA can up-regulate MIF expression and induce TNF-α and IL-8 secretion in THP-1 monocyte-derived macrophages.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Fenantrenos
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Arginina
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RNA Mensageiro
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Monócitos
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Diferenciação Celular
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Linhagem Celular
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Interleucina-8
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Fatores Inibidores da Migração de Macrófagos
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Fator de Necrose Tumoral alfa
Limite:
Humans
Idioma:
En
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Article