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Asymmetric dimethylarginine upregulates the expression of ACAT-1 in THP-1 macrophage-derived foam cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 2613-2618, 2010.
Artigo em Inglês | WPRIM | ID: wpr-267726
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of asymmetric dimethylarginine (ADMA) on ACAT-1 expression and cholesterol content in THP-1-derived macrophages and foam cells.</p><p><b>METHODS</b>THP-1 cells were induced to differentiate into macrophages and further into foam cells. The macrophages and foam cells were exposed to different concentrations (0, 3.75, 7.5, 15, and 30 µmol/L) of ADMA for varying time lengths (6, 12, and 24 h), and the changes in ACAT-1 mRNA and protein levels in the cells were measured with RT-PCR and Western blotting. The cellular cholesterol content was measured with enzyme-linked colorimetry assay.</p><p><b>RESULTS</b>In THP-1-derived macrophages and foam cells, the expression levels of ACAT-1 mRNA and protein and cellular cholesterol content increased significantly in response to ADMA treatment in a time- and concentration-dependent manner.</p><p><b>CONCLUSION</b>ADMA may play an important role in inducing foam cell formation from macrophages. ACAT-1 inhibition targeting the macrophages and foam cells may serve as a potential therapeutic target in the treatment of atherosclerosis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Acetil-CoA C-Acetiltransferase / Arginina / RNA Mensageiro / Monócitos / Regulação para Cima / Linhagem Celular / Colesterol / Biologia Celular / Células Espumosas Limite: Humanos Idioma: Inglês Revista: Journal of Southern Medical University Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Acetil-CoA C-Acetiltransferase / Arginina / RNA Mensageiro / Monócitos / Regulação para Cima / Linhagem Celular / Colesterol / Biologia Celular / Células Espumosas Limite: Humanos Idioma: Inglês Revista: Journal of Southern Medical University Ano de publicação: 2010 Tipo de documento: Artigo