Preliminary study on the development of germ cells from human fetal testicular tissues xenografted into the mouse / 中华男科学杂志
National Journal of Andrology
;
(12): 902-906, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-267785
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the development of xenografted primitive human germ cells by using fetal testicular tissues as donor tissues and an immunodeficient mouse as the recipient.</p><p><b>METHODS</b>Testicular tissue fragments of a 26-week fetus were grafted under the back skin of a castrated immunodeficient mouse. Grafts were taken out after 135 days and processed for morphological and histological analyses.</p><p><b>RESULTS</b>The mass of grafts grew from about 1 mm in diameter and 5 mg in wet weight to about 3 mm and more than 20 mg 135 days after grafting. Histological observations showed a significant expansion of seminiferous tubules after grafting (80 +/- 25 microm in diameter) in comparison with seminiferous cords at the time of grafting (60 +/- 15 microm in diameter). The seminiferous cords developed into seminiferous tubules with the epithelial border and lumen. After 135 days of grafting, most of the dispersedly distributed primitive Sertoli cells and germ cells migrated to the basal part of seminiferous epithelium, located on the basement membrane and few of germ cells differentiated into spermatogonia.</p><p><b>CONCLUSION</b>Human fetal testicular tissues could survive and continuously develop after being xenograft into castrated immunodeficient mice.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espermátides
/
Testículo
/
Transplante
/
Transplante Heterólogo
/
Transplante de Tecido Fetal
/
Biologia Celular
/
Camundongos Endogâmicos BALB C
/
Camundongos Nus
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2004
Tipo de documento:
Artigo
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