Post-thaw sperm functions of high- and low-fertility sperm donors: a comparative study of 40 cases / 中华男科学杂志
National Journal of Andrology
; (12): 30-36, 2014.
Article
em Zh
| WPRIM
| ID: wpr-267947
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate sperm function indexes that can be used to effectively evaluate the sperm donors' fertility so as to select healthy post-thaw semen samples and improve the success rate of assisted reproductive technology.</p><p><b>METHODS</b>According to the pregnancy outcomes, we divided 40 donor semen samples into a high-fertility group (n = 20) and a low-fertility group (n = 20). We measured and compared the concentration, progressive motility, morphology, acrosome intactness, DNA integrity and mitochondrial membrane potential (MMP) of the post-thaw sperm between the two groups.</p><p><b>RESULTS</b>There were statistically significant differences between the high- and low-fertility groups in the percentages of morphologically normal sperm ([18.50 +/- 6.10]% vs [14.42 +/- 6.44]%, P < 0.01), acrosome intactness ([86.17 +/- 4.49]% vs [80.04 +/- 7.52]%, P < 0.05) and DNA fragmentation index ([9.21 +/- 3.22]% vs [15.72 +/- 8.20]%, P < 0.05), but not in MMP ([56.75 +/- 18.80]% vs [52.23 +/- 18.86]%, P > 0.05). A significantly positive correlation was found between MMP and sperm motility (r = 0.760, P < 0.05), but not between other sperm functions and sperm concentration and motility.</p><p><b>CONCLUSION</b>Sperm concentration, motility, morphology, acrosome intactness rate and DNA integrity contribute effectively to the evaluation of the fertilization capacity of post-thaw donor semen samples.</p>
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Índice:
WPRIM
Assunto principal:
Fisiologia
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Preservação do Sêmen
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Contagem de Espermatozoides
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Motilidade dos Espermatozoides
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Espermatozoides
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Bancos de Esperma
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Criopreservação
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Fertilização
Limite:
Adult
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Female
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Humans
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Male
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Pregnancy
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2014
Tipo de documento:
Article