Construction of eukaryotic expression vector for VEGF165 gene and its expression in rat bladder smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 654-656, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-268055
ABSTRACT
<p><b>OBJECTIVE</b>To construct an eukaryotic expression vector for vascular endothelial growth factor (VEGF) 165 gene and obtain VEGF expression in rat bladder smooth muscle cells.</p><p><b>METHODS</b>VEGF165 cDNA was cloned into the eukaryotic expression vector pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/VEGF165 was transfected into the rat bladder smooth muscle cells by electroporation. VEGF expression in the cells was determined by RT-PCR and immunofluoresence assay, and the biological activity of VEGF in the supernant of the transinfected cell culture was tested by MTT assay.</p><p><b>RESULTS AND CONCLUSION</b>VEGF expression was obtained in the transinfected cells, and the supernant of the transinfected cell cultures stimulated the proliferation of the endothelial cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Bexiga Urinária
/
Transfecção
/
Expressão Gênica
/
Linhagem Celular
/
Células Cultivadas
/
Imunofluorescência
/
Clonagem Molecular
/
Meios de Cultivo Condicionados
/
DNA Complementar
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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